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cd63  (Miltenyi Biotec)
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Miltenyi Biotec cd63
Cd63, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd42b gpibα antihuman polyclonal antibody  (R&D Systems)
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R&D Systems cd42b gpibα antihuman polyclonal antibody
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Cd42b Gpibα Antihuman Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd42b gpibα antihuman polyclonal antibody/product/R&D Systems
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cd42b  (Miltenyi Biotec)
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Miltenyi Biotec cd42b
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Cd42b, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd42b/product/Miltenyi Biotec
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anti cd42b pe  (Miltenyi Biotec)
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Miltenyi Biotec anti cd42b pe
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Anti Cd42b Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd42b pe/product/Miltenyi Biotec
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anti cd42b pe - by Bioz Stars, 2026-03
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phycoerythrin- cyanin 5 (pe- cy5)- conjugated mouse anti- human cd42b antibody  (Becton Dickinson)
90
Becton Dickinson phycoerythrin- cyanin 5 (pe- cy5)- conjugated mouse anti- human cd42b antibody
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Phycoerythrin Cyanin 5 (Pe Cy5) Conjugated Mouse Anti Human Cd42b Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd42b  (Miltenyi Biotec)
94
Miltenyi Biotec anti cd42b
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Anti Cd42b, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd42b/product/Miltenyi Biotec
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phycoerythrin anti cd42b  (Miltenyi Biotec)
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Miltenyi Biotec phycoerythrin anti cd42b
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Phycoerythrin Anti Cd42b, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human cd42b-pe antibody  (Thermo Fisher)
90
Thermo Fisher anti-human cd42b-pe antibody
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Anti Human Cd42b Pe Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd42b antibody, anti-human, reafinity  (Miltenyi Biotec)
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Miltenyi Biotec cd42b antibody, anti-human, reafinity
( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner <t>GPIbα</t> on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.
Cd42b Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner GPIbα on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.

Journal: Toxins

Article Title: A Novel P-III Metalloproteinase from Bothrops barnetti Venom Degrades Extracellular Matrix Proteins, Inhibits Platelet Aggregation, and Disrupts Endothelial Cell Adhesion via α5β1 Integrin Receptors to Arginine–Glycine–Aspartic Acid (RGD)-Containing Molecules

doi: 10.3390/toxins16110486

Figure Lengend Snippet: ( A ) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. ( B ) Bar-III does not cleave the vWF receptor partner GPIbα on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIbα expression on platelets. ( C ) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item.

Article Snippet: The GPVI polyclonal antibody (AF3627) of human platelets and CD42b/GPIbα (antihuman) polyclonal antibody (AF4067) were acquired from R&D Systems (Minneapolis, MN, USA).

Techniques: Western Blot, Recombinant, SDS Page, Incubation, Expressing, Control